Feng Chen

Biography:

I was born in the year of dragon. I like noodles and sashimi (sushi is OK, I will leave the rice). I was interested in marine science because of large and beautiful sea mammals. However, I am not stuck with the smallest “living creatures” in the sea – viruses. I can see these tiny creatures with my SYGR Gold approach, but they really hurt my eyes…

Research Focus:

Ecological interaction among marine viruses, bacteria and phytoplankton; phylogenetic diversity and molecular evolution of marine microbes; functional genomics and environmental proteomics.

Publications:

Chen*, F., K. Wang, J. Stewart, R. Belas. 2006. Induction of prophages from a marine reseobacterium, Silicibacter sp. TM1040: A genomic approach. Appl. Environ. Microbiol. 72:4995-5001.

Kan, J., B. Crump, K. Wang, and F. Chen*. 2006. Bacterioplankton community in Chesapeake Bay: Predictable or random assemblages. Limnol. Oceanogr. (in press).

Chen*, F., K. Wang, J, Kan, and M. Suzuki, E. Wommack. 2006. Diverse and unique picocyanobacteria found in the Chesapeake Bay. Appl. Environ. Microbiol. 72:2239-2243.

Kan, J., T. E. Hanson, J. M. Ginter, K. Wang, F. Chen*. 2005. Metaproteomic analysis of Chesapeake Bay microbial communities. Saline Systems 1:7.

Kan, J., K. Wang, and F. Chen*. 2005. Temporal variation and detection limit of an estuarine bacterioplankton community analyzed by denaturing gradient gel electrophoresis (DGGE). Aquat. Microb. Ecol. 42:7-18.

Bettarel, Y., J. Kan, K. Wang, S. Cooney, K. Williamson, F. Chen, E. Wommack, W. Coats. 2005. Isolation and characterisation of a small nuclear inclusion virus infecting the diatom Chaetoceros c.f. gracilis. Aquat. Microbial. Ecol. 40:103-114.

Lohr, J. E., F. Chen, and R. T. Hill. 2005. Genomic analysis of bacteriophage JL001: Insights into interaction with a sponge-associated alpha-proteobacterium. Appl. Environ. Microbiol. 71: 1598-1609.

Chen, F*., K. Wang, J. Kan, D.S. Bachoon, J. Lu, S. Lau, and L. Campbell. 2004. Phylogenetic diversity of Synechococcus in the Chesapeake Bay revealed by Ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) large subunit gene (rbcL) sequences. Aquatic Microbial Ecology. 36:153-164

Kan, J. and F. Chen*. 2004. Co-monitoring bacterial and dinoflagellates communities by denaturing gradient gel electrophoresis (DGGE) and SSU rDNA sequencing during a dinoflagellates bloom. Acta Oceanologia Sinica. 23 (3):483-492.

Wang, K. and F. Chen*. 2004. Genetic Diversity and Population Dynamics of Cyanophage Communities in the Chesapeake Bay. Aquatic Microbial Ecology 34:105-116, 2004.

Chen, F*. and Lu, J. R. 2002. Genomic Sequence and Evolution of Marine Cyanophage P60: a new Insight on Lytic and Lysogenic Phages. Appl. Environ. Microbiol. 68:2589-2594.

Zhong, Y., F. Chen*, J. R. Lu, L. Poorvin, S. Wilhelm, R. Hodson. 2002. Phylogenetic diversity of marine cyanophage isolates and natural virus communities as revealed by sequences of viral capsid assembly protein gene g20. Appl. Environ. Microbiol. 68:1576-1584.

Lau, S. C. K., K. K. W. Mak, F. Chen and P. Y. Qian. 2002. Bioactivity of bacterial strains isolated from marine biofilms in Hong Kong waters for the induction of larval settlement in the marine polychaete Hydroides elegans. Mar. Ecol. Prog. Ser. 226:301-310.

Bachoon, D., F. Chen, and R. E. Hodson. 2001. RNA recovery and detection of mRNA by RT-PCR from preserved prokaryotic samples. FEMS Microbiol. Lett. 201:127-132.

Lu, J.R., F. Chen* and R. E. Hodson. 2001. Distribution, isolation, host specificity and diversity of marine cyanophages infecting Synechococcus spp. in Coastal Estuary. Appl. Environ. Microbiol. 67:3285-3290.

Chen, F.*, J. R. Lu, B. Binder and R. E. Hodson. 2001. Enumeration of viruses in aquatic environments using SYBR Glod stain: application of digital image analysis and flow cytometer. Appl. Environ. Microbiol. 67:539-545.

Chen, F.*, B. Binder, R. E. Hodson. 2000. Flow cytometric detection of specific gene expression in prokaryotic cells using in situ RT-PCR. FEMS Microbiology Letters. 184:291-295.

Chen, F.*, W. A. Dustman and R. E. Hodson. 1999. Detection of toluene dioxygenase gene and gene expression in Pseudomonas putida F1 in a toluene exposed seawater using in situ PCR and hybridization. Hydrobiologia 401:131-138.

Chen, F.*, J. M. Gonzalez, W. Dustman, M. Moran and R. Hodson. 1997. In situ reverse transcription: a new molecular approach to study microbial community structure. Appl. Environ. Microbiol. 63 (12):4907-4913.

Chen, F.*, S. M. Short and C. A. Suttle. 1996. Genetic diversity in marine algal virus communities as revealed by sequence analysis of DNA polymerase Genes. Appl. Environ. Microbiol. 62 (8): 2869-2874.

Chen, F., and C. A. Suttle. 1996. Evolutionary relationships among large double-stranded DNA viruses that infect microalgae and other organisms as inferred from DNA polymerase genes. Virology 219:170-178.

Chen, F., and C. A. Suttle. 1995. Amplification of DNA polymerase gene fragments from viruses infecting microalgae. Appl. Environ. Microbiol.. 61 (4):1274-1278.

Chen, F., and C. A. Suttle. 1995. Nested PCR with three highly degenerate primers for amplification and identification of DNA from related organisms". BioTechniques. 18 (4): 609-612.

Suttle, C. A., and F. Chen. 1992. Mechanisms and rates of decay of marine viruses in seawater. Appl. Environ. Microbiol.. 58 (11): 3721-3729.

Chen, F., B. Reid, and C. R. Arnold. 1990. Maturation and spawning of the white shrimp Penaeus vannamei Boore in a recirculating system. Journal of World Aquaculture, 22(3): 167-172, 1991.

Book chapters

Chen, F.* and R. E. Hodson. 2001. In situ PCR/RT-PCR coupled with in situ hybridization for detection of functional gene and gene expression in prokaryotic cells. In: J. Paul (ed.) Methods in Marine Microbiology. pp.409-424. Academic Press.

Chen, F.*, W. A. Dustman, R. E. Hodson. 2000. Application of in situ reverse transcription to estuarine bacterial community analysis. In: C. R. Bell, M. Brylinsky, P. Johnson-Green (ed.), Microbial Biosystems: New Frontiers. Proceedings of the 8th International Symposium on Microbial Ecology. Atlantic Canada Society for Microbial Ecology. Halifax, Canada.

Chen, F.*, and R. E. Hodson. 1999. Viewing microbes with in situ molecular approaches, a Mini Review. In: H. S. Xu and R. Colwell (ed.), Proceedings in Marine Biotechnology, pp. 300-305, China Ocean Press, Beijing.

Chen, F.*, W. Dustman, M. A. Moran, and R. E. Hodson. 1998. In situ PCR methodologies for visualization of microscale genetic and taxonomic diversities of prokaryotic communities. ch. 3.3.9. In: A. D. L. Akkermans, J. D. van Elsas, F. J. DeBruijn (ed.), Molecular Microbial Ecology Manual. Kluwer Acadamic Publishers, The Netherland.

Chen, F., S. M. Short and C. A. Suttle. (1996) Sequence analysis indicates high genetic diversity in marine algal virus communities. Eos 76 (3 suppl): OS207.

Suttle, C. A., A. M. Chan, F. Chen, and D. R. Garza, 1993. Cyanophages and sunlight: a paradox. In: Trends in Microbial Ecology, eds. R. Guerrero & C. Pedros-Alio. pp303-307.

Suttle, C. A., F. Chen, and A. M. Chan (1992). Marine viruses: decay rates, diversity and ecological implications. In: Nash, C. C. II (ed.) International Marine Biotechnology Conference 'IMBC-91': Short Communications of the Invited Lectures Developments in Microbiology. W. Brown Co., Dubuque, IA, p. 153-163.

Chen, F., and G. Y. Wang. 1988. Relationship between vertical distribution subsurface chlorophyll a and the seawater stability in the western tropical Pacific Ocean, p. 485-492. In: J. P. Chao and J. A. Young (ed.), Air-Sea Interaction in Tropical Western Pacific, China Ocean Press.

Wang, G. Y., and F. Chen. 1988. Study on ecological structure feature in the western tropical Pacific, p. 493-500. In: J. P. Chao and J. A. Young (ed.), Air-Sea Interaction in Tropical Western Pacific, China Ocean Press.

Presentations:

• Chen, F., 2006. Characterization of conserved genes for virus group. The Scientific Committee for Oceanographic Research (SCOR)-working group on marine viruses. June 1-3, 2006, The University of British Columbia, Vancouver, BC. (invited speech).

• Wang, K., K. Omara, J. Kan, and F. Chen. 2006. Estuarine cyanophages and their psbA gene. The Scientific Committee for Oceanographic Research (SCOR)-working group on marine viruses. June 1-3, 2006, The University of British Columbia, Vancouver, BC. (poster)

• Chen, F., K. Wang, J. Stewart, R. Belas. 2006. Induction of multiple prophages from a marine Roseobacterim, a genomic approach. American Society of Limnology and Oceanography Summer Meeting, June 5-9, 2006, Victoria, Canada.

• Kan, J., T. Hanson, C. Cary, E. Wommack, R. Hill, F. Chen. 2006. Community proteomics, a new way to explore microbial functions in natural environments. American Society of Limnology and Oceanography Summer Meeting, June 5-9, 2006, Victoria, Canada.

• Wang, K., and F. Chen. 2006. Seasonal successions and genetic diversities of estuarine Synechococcus and their co-occurring phages: A three-year survey in Chesapeake Bay. American Society of Limnology and Oceanography Summer Meeting, June 5-9, 2006, Victoria, Canada.

• O’Mara, K. J., K. Wang, J. Kan, S. Bench, T. Hanson, E. Wommack, F. Chen. 2006. Genetic diversity and seasonal patterns of cyanophage psbA gene in the estuarine environment revealed by a newly designed PCR primer set. American Society of Limnology and Oceanography Summer Meeting, June 5-9, 2006, Victoria, Canada.

• Eissler, Y., K. Wang, F. Chen, W. Coats. 2006. Molecular characterization of Chaetoceros cf gracilis virus isolated from Chesapeake Bay, USA. American Society of Limnology and Oceanography Summer Meeting, June 5-9, 2006, Victoria, Canada.

• Crump, B.C., H. E. Adams, F. Chen, J. E. Hobbie, J. Kan, and G. W. Kling. 2006. Synchrony and seasonality in bacterioplankton community composition. 11th International Symposium on Microbial Ecology (ISME-11), Vienna, Austria, August 20-25, 2006.

Kan, J., T. Hanson, B. Campbell, C. Cary, E. Wommack, R. Hill, and F. Chen. Meta-proteomics, a new way to explore microbial function in natural environments. HUPO 4th Annual World Congress, August 28-September 1, 2005. Munich, Germany (Poster). The abstract was published in Molecular and Cellular Proteomics 4.8 (Suppl. 1):S286

Wang, K. and F. Chen. Isolation and Characterization of Cyanophages of Marine Cluster B Synechococcus. ASLO summer meeting June 18-24, 2005. Santiago de Compostela, Spain. Oral presentation.

Wang, K. and F. Chen. Characterization of cyanophages infecting estuarine Synechococcus spp. International Marine Biotechnology Conference, June 7-12, 2005, St. John’s, Newfoundland, Canada. Oral Presentation.

Chen, F., K. Wang, J. Kan, and M. Suzuki. Synechococcus ecotypes in Chesapeake Bay revealed by the RuBisCO gene and ITS sequences. International Marine Biotechnology Conference, June 7-12, 2005, St. John’s, Newfoundland, Canada. Oral Presentation.

Kan, J., T. Hanson, K. Wang, B. Campbell, C. Cary, E. Wommack, R. Hill, and F. Chen. Meta-proteomics, a New Way to Explore Microbial Processes in the Ocean? International Marine Biotechnology Conference, June 7-12, 2005, St. John’s, Newfoundland, Canada. Oral Presentation.

Chen, F., K. Wang, J. Kan, D. Bachoon, L. Campbell. Phylogenetic Diversity of Estuarine Synechococcus Revealed by Ribulose-1,5-Bisphophate Carboxylase-Oxygenase Large Subunit Gene Sequences. The 10th International Symposium on Microbial Ecology, ISME-10. August 22-27, 2004. Cancun, Mexico. Poster presentation.

Chen, F., and J. Kan. A proteomic study of picoplankton community. The 10th International Symposium on Microbial Ecology, ISME-10. August 22-27, 2004. Cancun, Mexico. Oral presentation.

Kan, J., M. Suzuki, K. Wang, and F. Chen. Spatial and temporal variation of Chesapeake bacterioplankton communities revealed by DGGE and clone libraries. The 10th International Symposium on Microbial Ecology, ISME-10. August 22-27, 2004. Cancun, Mexico. Poster presentation.

Kan, J., and F. Chen. Are 5000 bacterial cells per ml the detection threshold for DGGE analysis? The 10th International Symposium on Microbial Ecology, ISME-10. August 22-27, 2004. Cancun, Mexico. Poster presentation.

Wang, K., J. Kan, and F. Chen. Co-monitoring Synechococcus and synechophage in the Chesapeake Bay. The 10th International Symposium on Microbial Ecology, ISME-10. August 22-27, 2004. Cancun, Mexico. Poster presentation.

Suzuki, M.T., J. Kan, F. Chen and S. E. Evans. Fast Screening and analysis of Chesapeake Bay bacterioplankton rIBOSOMAL RNA operon clone libraries. The 10th International Symposium on Microbial Ecology, ISME-10. August 22-27, 2004. Cancun, Mexico (poster presentation).

Chen, F. and K. Wang. Population Dynamics and Genetic Diversity of Synechococcus in the Chesapeake Bay. Gordon Research Conference on Marine Microbes: Picophytoplankton, from Ecology to Genomics, June 6-10, Roscoff, Brittany, France. Poster presentation.

Isaacs, L. T., J, Kan, S. Sidiqqi, D. Horn, F. Chen, T. Wright, and J. Enticknap, and R. Hill. Analysis of the Microbial Community of the Chesapeake Redbeard Sponges, Microciona prolifera. The 104th General Meeting of American Society of Microbiology. May, 2004, New Orleans. Poster presentation.

Wommack, K. E. and F. Chen. Diversity and community composition of virioplankton over an annual biological cycle of the Chesapeake Bay. Ocean Research Conference, February 15-20, 2004, Hawaii. Oral presentation.

Chen, Feng and Kui Wang. Phylogenetic Relationship of Estuarine Synechococcus spp. Revealed by the RubisCO Gene. Marine Biotechnology Conference September 21-27, 2003, Chiba, Japan. Oral presentation.

Kan, Jingjun and F. Chen. Genetic fingerprinting and proteomic analysis of bacterial communities in the Chesapeake Bay. Marine Biotechnology Conference September 21-27, 2003, Chiba, Japan. Oral presentation.

Wang, Kui and F. Chen. Isolation and characterization of Synechococcus spp. and their phages from Chesapeake Bay. Marine Biotechnology Conference September 21-27, 2003, Chiba, Japan. Poster presentation.

Wommack, K. E., Kurt E. Williamson, Danielle Winget, Rebekah R. Helton, Shellie Bench, F. Chen, Kui Wang, Jinjun Kan, D. W. Coats, Sean Cooney, and Yvan Bettarel. Microbial Observatory for Virioplankton Ecology (MOVE): The role of viruses in the annual biological cycle of the Chesapeake Bay. NSF Microbial Observatories Workshop, Arlington, VA, September 15-16, 2003. Poster presentation.

Chen, F. and J.J. Kan. Environmental microbial proteomics: linking microgeochemial functions. The 103th General Meeting of American Society of Microbiology. May 18-22, 2003, Washington DC. Poster presentation.

Wang, K. and F. Chen. Genetic diversity and population dynamics of Synechococcus spp. and cyanophages in the Chesapeake Bay. The 103th General Meeting of American Society of Microbiology. May 18-22, 2003 Washington DC. Poster presentation.

Kan, J.J. and F. Chen. Spatial and temporal dynamics of bacterial communities in the Chesapeake Bay. The 103th General Meeting of American Society of Microbiology. May 18-22, 2003, Washington DC. Poster presentation.

Wang, K. and F. Chen. Genetic diversity of virus that infect marine Synechococcus in the Chesapeake Bay. MEES colloquium, College Park. Nov. 2-3, 2001. Poster presentation.

Chen, F., Y. Zhong, J. Lu, L. Poorvin, S. Wilhelm, and R. Hodson. What do we learn from gene and genomic sequences of cyanophages? The 9th International Symposium of Microbial Ecology. Amsterdam, August 26-31. Oral presentation.

Chen, F., J. Lu, Y. Zhong, R. E. Hodson. Distribution, diversity and genomic sequence of cyanophages infecting marine Synechococcus spp. The 101th general meeting of the American Society for Microbiology, Orlando, May 20-24, 2001. Poster presentation.

Chen, F., B. Binder, R. E. Hodson. Flow cytometric detection of specific gene expression in prokaryotic cells using in situ RT-PCR. DOE BI-OMP Workshop, March 30-31, 2001, Savannah, Georgia. Poster presentation.

Chen, F. and R. E. Hodson. Marine cyanophages: distribution, phenotypes, genotypes, phylogeny and genomic sequencing. The 5th International Marine Biotechnology Conference, September- October, 2000. Townsville, Queensland, Australia. Oral presentation.

Hodson, R. E. and F. Chen. Visualizing Marine Microbes with in situ Molecular Approaches (an overview). The 5th International Marine Biotechnology Conference, September- October, 2000. Townsville, Queensland, Australia. Oral presentation.

Wommack, K. E., F. Chen, and R. E. Hodson. Marine diazotroph ecology: development of a molecular genetic in situ detection method. The 5th International Marine Biotechnology Conference, September- October, 2000. Townsville, Queensland, Australia. Poster presentation.

Chen, F., Y. Zhong, D. Bachoon, J. R. Lu, S. C. K. Lau, L. Campbell and R. E. Hodson. Cross infectivity of marine viruses unveiled by genetic linkage of their hosts. American Society of Limnology and Oceanography-2000, Copenhagen, Denmark, 5-9 June, 2000. Oral presentation.

Hodson, R. E. and F. Chen, In situ activity and functional diversity of microbes linking carbon and nitrogen cycles in marine ecosystems. Aquatic Science Meeting, ASLO, June 5-9, 2000. Copenhagen, Denmark. Oral presentation.

Lu, J.R., F. Chen and R. E. Hodson. Genomic sequence of a lytic cyanophage of Synechococcus spp. The 100th General Meeting of the American Society for Microbiology, Los Angeles, May 21-25, 2000. Poster presentation.

Zhong, Y., F. Chen, and R. E. Hodson. Phyletic diversity of marine cyanophages as revealed by viral capsid assembly protein gp 20. The 100th General Meeting of the American Society for Microbiology, Los Angeles, May 21-25, 2000. Poster presentation.

Bachoon, D., F. Chen and R. E. Hodson. RNA recovery and detection of mRNA by RT-PCR from stored prokaryotic samples. The 100th General Meeting of the American Society for Microbiology, Los Angeles, May 21-25, 2000. Poster presentation.

Hodson, R.E. and F. Chen. In situ Activity & Functional Diversity of Microbes Linking Carbon & Nitrogen Cycles in Marine Ecosystems. DOE BI-OMP Workshop, March 15-16, 2000, Tallahassee, Florida. Poster presentation.

Chen, F., D. Bachoon, S. C. K. Lau, J. R. Lu, L. Campbell and R. E. Hodson. Coastal Synechococcus spp. are much more complex than oceanic Synechococcus spp. DOE BI-OMP Workshop, March 15-16, 2000, Tallahassee, Florida. Poster presentation.

Sullivan, J., F. Chen and R. H. Hodson. Toward the application of prokaryotic in situ PCR to the study of denitrification. DOE BI-OMP Workshop, March 15-16, 2000, Tallahassee, Florida. Poster presentation.

Dustman, W. A., F. Chen and R. E. Hodson. Filter concentration of laboratory bacterial cultures and natural seawater communities: an adaptation for analysis of genetic and functional diversity by in situ PCR, in situ RT-PCR and in situ reverse transcription. DOE BI-OMP Workshop, March 15-16, 2000, Tallahassee, Florida. Poster presentation.

Bachoon, D., F. Chen and R. E. Hodson. RNA recovery and detection of mRNA by RT-PCR from preserved prokaryotic samples. DOE BI-OMP Workshop, March 15-16, 2000, Tallahassee, Florida. Poster presentation.

Wommack, K. E., F. Chen and R. E. Hodson. A rapid method for obtaining the complete nucleotide coding sequence for nifH. DOE BI-OMP Workshop, March 15-16, 2000, Tallahassee, Florida. Poster presentation.

Dudeck, K. L., F. Chen and R. E. Hodson. PCR amplification of the amoA gene from two marine ammonium-oxidizing bacteria, Nitrosomonas cryotolerans and Nitrosococcus oceani DOE BI-OMP Workshop, March 15-16, 2000, Tallahassee, Florida. Poster presentation.

Chen, F., J. R. Lu, Y. Zhong, and R. E. Hodson. Marine cyanophages reveal a high phenotypic and genotypic diversity. The 99th General Meeting of the American Society for Microbiology, Chicago, IL., May 30-June 3, 1999. Poster presentation.

Chen, F., J. R. Lu, and R. E. Hodson. Enumeration of viruses in aquatic environemnts using SYBR Glod stain and digital image analysis. The 99th General Meeting of the American Society for Microbiology, Chicago, IL., May 30-June 3, 1999. Poster presentation.

Dustman, W. A., F. Chen, and R. E. Hodson. Filter concentration of natural seawater microbial communities and analysis of genetic and functional diversity by in situ reverse transcription (ISRT) and in situ RT-PCR. The 99th General Meeting of the American Society for Microbiology, Chicago, IL., May 30-June 3, 1999. Poster presentation.

Williams, K. L., F. Chen, W. A. Dustman, and R. E. Hodson. PCR amplification of the amoA gene from a marine ammonium-oxidizing bacterium Nitrosomonas cryotolerans. The 99th General Meeting of the American Society for Microbiology, Chicago, IL., May 30-June 3, 1999. Poster presentation.

Chen, F., J. R. Lu, and R. E. Hodson. Distribution of cyanophages and total viruses along Georgia coastal rivers. ASLO meeting, Feb. 1-5. 1999, Santa Fe, New Mexico. Poster presentation.

Chen, F., J. Lu and R. E. Hodson. Molecular phylogeny and in situ mRNA detection of the rbcL gene in Marine Synechococcus spp. ASLO meeting, Feb. 1-5. 1999, Santa Fe, New Mexico. Poster presentation.

Wommack, E., F. Chen and R. E. Hodson. Nitrogenase in marine Vibrio. ASLO meeting, Feb. 1-5. 1999, Santa Fe, New Mexico. Poster presentation.

Sullivan, J. B., F. Chen and R. E. Hodson. Constitutive production of nitrite reductase in Pseudomonas stutzeri detected by in situ RT-PCR. ASLO meeting, Feb. 1-5. 1999, Santa Fe, New Mexico. Poster presentation.

Hodson, R. E., F. Chen and W. A. Dustman. Application of in situ reverse transcription to estuarine bacterial community analysis. ASLO meeting, Feb. 1-5. 1999, Santa Fe, New Mexico. Oral presenation.

Chen, F. and R. E. Hodson. Viewing microbes with in situ molecular approaches. International Symposium on Marine Biotechnology (ISPPMB’98). Oct. 6-9, Qingdao, China. Oral presentation.

Chen, F., J. R. Lu, and R. E. Hodson. RuBisCO genes reveal a close relationship between Synechococcus strains WH7803 and WH8101. The 8th International Symposium on Microbial Ecology, Aug. 9-14, 1998, Halifax, Canada. Poster presentation.

Hodson, E. and F. Chen. In situ nucleic acid amplification for studies of estuarine bacterial activity and diversity. The 8th International Symposium on Microbial Ecology, Aug. 9-14, 1998, Halifax, Canada. Oral presentation.

Dustman, W. A., F. Chen and R. E. Hodson. Characterizing genetic diversity and activity of filter-concentrated microorganisms using a modified in situ reverse transcription protocol. The 8th International Symposium on Microbial Ecology, Aug. 9-14, 1998, Halifax, Canada. Poster presentation.

Chen, F. and R. E. Hodson. Detection of carbon and nitrogen utilizing genes (rbcL and amoA) and their expression in bacteria using in situ PCR/hybridization methods. The 98th General Meeting of the American Society for Microbiology, Atlanta, Georgia, 1998. Poster presentation.

Chen, F., W. A. Dustman, M. A. Moran and R. E. Hodson. Using prokaryotic in situ PCR to examine the todC1 gene and its expression in a toluene degrading bacterium in marine microcosms. The 97th General Meeting of the American Society for Microbiology, Miami Beach, Florida, 1997. Poster presentation.

Hodson, R. E., M. A. Moran, F. Chen, W. Dustman, and R. Garg. Amplified in situ taxonomic identification and detection of specific genes and their expression in marine bacterial population. ASLO 97 Aquatic Sciences Meeting. Santa Fe, New Mexico, February, 10-14, 1997. Poster presentation.

Chen, F., J. M. González, W. A. Dustman, M. A. Moran and R. E. Hodson. In situ reverse transcription, an approach to characterize genetic diversity (based on 16S rRNA) and activity (based on mRNA) of prokaryotes. ASLO 97 Aquatic Sciences Meeting. Santa Fe, New Mexico, February, 10-14, 1997. Oral presentation.

Chen, F., S. M. Short and C. A. Suttle. Sequence analysis indicates high genetic diversity in marine algal virus communities. Ocean Sciences Meeting, San Diego, California, February, 12-16, 1996. Poster presentation.

Chen, F. and C. A. Suttle. Phylogeny of large double-stranded DNA viruses which infect microalgae, as inferred from DNA polymerase gene sequences. The 14th annual meeting of American Society for Virology, Austin, Texas, July 8-12, 1995. Poster presentation.

Chen, F., M. T. Cottrell and C. A. Suttle. DNA polymerase reflects genomic relatedness among algal viruses. The 95th General Meeting of the American Society for Microbiology, Washington, D. C., May 21-25, 1995. Poster presentation.

Suttle, C. A., F. Chen and M. T. Cottrell. DNA polymerase genes as probes of the diversity and phylogeny of marine microbial populations. Keystone Symposia on Molecular and Cellular Biology, Santa Fe, New Mexico, March 5-11, 1995. Poster presentation.

Chen, F., M. T. Cottrell and C. A. Suttle. Development of a PCR-based technique for detection and quantifying algal viruses in aquatic environments. ALSO/PSA meeting, Miami, Florida, June 12-16, 1994. Poster presentation.

Chen, F., B. Reid and C. R. Arnold. Maturation and spawning of the white shrimp Penaeus vannamei Boore in a recirculating system. World Aquaculture Society Conference, Halifax, Nova Scotia, June 10-14, 1990. Oral presentation.

Invited presentations:

“Population dynamics of virio- and bacterio-plankton in the Chesapeake Bay” May, 4, 2004. Graduate College of Marine Studies, University of Delaware, Lewes Campus.

“Interaction between virus and host in the coastal ecosystem” March 15, 2004. College of Life Science, Zhong Shan University, Guanzhou, China.

“Spatial and temporal variations of bacterioplankton community in the Chesapeake Bay” March 10, 2004. Atmospheric, Marine and Coastal Environment (AMCE) Program, Hong Kong University of Science and Technology, Hong Kong

“Distribution and Diversity of Synechococcus and their Phages in the Chesapeake Bay”. April, 2003. Horn Point Laboratory, UMCES.

“Exploring Microbial Diversity and Function in the Sea”. Hong Kong University of Science and Technology. Nov. 20, 2002.

Chen, F. Series lectures and seminars on microbial ecology. Xiamen University, China. Nov. 13-19, 2002.

“Unexpected Genetic Diversity found in Marine Virus Communities”. ASLO Summer Meeting in Victoria, Canada. June 10-15, 2002.

“Genomic Evolution of Lytic and Lysogenic Phages”. ASLO/AGU Ocean Science Meeting in Hawaii. February 11-15, 2002.

"Exploring Microbial Diversity and Function in the Sea", Department of Biology, University of Alabama, 2000.

"In situ Molecular Approaches for Monitoring Gene Expression in Prokaryotes" Department of Biology and Biochemistry. University of Colorado. 1999.

“ Molecular Tools to Study Genetic Diversity and Activity of Marine Microbes”. June 29, 1999, Division of Microbiology, National Research Center of Biotechnology, GBF, Braunschweig, Germany.

“Viewing Marine Microbes with in situ Molecular Approaches”. Max Planck Institute for Marine Microbiology. June 25, 1999, Bremen, Germany.

“Genetic Diversity of Cyanophages Infecting Marine Synechococcus spp.” University of Rostock, June 23, 1999, Rostock, Germany.

“Application of in situ PCR and RT-PCR to Study Genetic Diversity and Function of Marine Microbes”. University of Rostock, June 18, 1999, Rostock, Germany.

"Exploring Microbial Diversity of Function in Marine Microbes", Department of Biology, University of New Mexico, Albuquerque, New Mexico. 1999.

"In situ Molecular Detection: A Way to study Microbial Function" Department of Biological Sciences, California State University, Long Beach, 1999.

"Molecular Characterization of Viruses infecting Marine Algae", Department of Biology, The University of Akron. 1998.

"Phylogenetic Relationship of Algal Viruses" Federated Department of Biological Sciences, New Jersey Institute of Technology, 1997

"In situ PCR for Visualizing Gene Expression in Marine Bacteria". Department of Biology, The University of West Florida, Pensacola, FL. 1997

“PI-PCR, A Potential Tool for LMER Projects”. The Land-Margin Research Program Annual Meeting, Savannah, Georgia, November 7-9, 1996.

“Marine Viruses and their Ecological Roles”. Institute of Ecology, University of Georgia, January 10, 1996.

Assistant Professor

University of Maryland Biotechnology Institute

Center of Marine Biotechnology

701 East Pratt Street

Baltimore, MD 21202

Phone: 410-234-8866

Lab: 410-234-8867

Fax: 410-234-8896

Email: chenf_at_umbi.umd.edu

Education

Ph.D., 1995, The University of Texas at Austin, Autstin, TX (the Longhorn!)

M.S., 1988, First Institute of Oceanography, China

B.S., 1985, Ocean University of China

Websites: